Composite
Part:BBa_K4800055:Design
Designed by: Yifei Wu Group: iGEM23_NJTech-China-B (2023-10-11)
pRSFDuet-sfp-MmCAR-YahK
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4145
Illegal XbaI site found at 1415
Illegal PstI site found at 4580
Illegal PstI site found at 4607
Illegal PstI site found at 5876
Illegal PstI site found at 5903
Illegal PstI site found at 6296
Illegal PstI site found at 6792
Illegal PstI site found at 7436 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4145
Illegal NheI site found at 7704
Illegal PstI site found at 4580
Illegal PstI site found at 4607
Illegal PstI site found at 5876
Illegal PstI site found at 5903
Illegal PstI site found at 6296
Illegal PstI site found at 6792
Illegal PstI site found at 7436 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4145
Illegal BglII site found at 5134
Illegal BglII site found at 5760
Illegal BglII site found at 6036
Illegal BglII site found at 6727
Illegal BamHI site found at 4117
Illegal XhoI site found at 7676 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4145
Illegal XbaI site found at 1415
Illegal PstI site found at 4580
Illegal PstI site found at 4607
Illegal PstI site found at 5876
Illegal PstI site found at 5903
Illegal PstI site found at 6296
Illegal PstI site found at 6792
Illegal PstI site found at 7436 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4145
Illegal XbaI site found at 1415
Illegal PstI site found at 4580
Illegal PstI site found at 4607
Illegal PstI site found at 5876
Illegal PstI site found at 5903
Illegal PstI site found at 6296
Illegal PstI site found at 6792
Illegal PstI site found at 7436
Illegal NgoMIV site found at 6404
Illegal NgoMIV site found at 6466
Illegal NgoMIV site found at 6683
Illegal NgoMIV site found at 7130
Illegal AgeI site found at 4310
Illegal AgeI site found at 5321
Illegal AgeI site found at 5618
Illegal AgeI site found at 6503
Illegal AgeI site found at 6512
Illegal AgeI site found at 7187
Illegal AgeI site found at 9049 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 9122
Illegal SapI site found at 660
Design Notes
None.
Source
None.